polyclonal rabbit anti human abca1 antibody  (Novus Biologicals)

Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Inhibition of miR-873 Provides Therapeutic Benefit in a Lipopolysaccharide-Induced Neuroinflammatory Model of Parkinson's Disease

doi: 10.1155/2020/8735249

Figure Lengend Snippet: The effects of the miR-873 inhibitor on the damage to DA neurons in the substantia nigra pars compacta in a LPS-induced rat model of PD. The animals were transfected with the miR-873 sponge 3 days before LPS treatment or 8 and 16 days after LPS treatment (a). The damage to DA neurons following LPS treatment was detected by immunohistochemistry staining ( n = 5) (b). The reduction in the tyrosine hydroxylase- (TH-) positive cells on the lesioned side was attenuated in the rats transfected with the miR-873 sponge 3 days before LPS treatment or 8 days after LPS injection, compared with LPS treatment alone (c). The accumulation of α -synuclein in DA neurons was examined by fluorescence immunohistochemistry ( n = 5) (d and e). The number of apomorphine-induced rotations following LPS treatment was decreased in the rats treated with the miR-873 sponge compared with the rats treated with LPS alone ( n = 10) (f). The mRNA levels of miR-873 were increased by LPS treatment, compared with the control ( n = 5) (g). Transfection of the miR-873 sponge attenuated the inhibition of the mRNA levels of ABCA1 (h) and A20 (i) following LPS treatment. The data are expressed as the mean ± S.D.; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 compared with the controls.

Article Snippet: These membranes were incubated with a polyclonal rabbit anti-human ABCA1 antibody (1 : 1000, NB400-105, Novus Biologicals, Colorado, USA) at 4°C overnight and then incubated with a biotinylated anti-rabbit IgG antibody (1 : 100, Vector Laboratories, Burlington, Canada).

Techniques: Transfection, Immunohistochemistry, Staining, Injection, Fluorescence, Control, Inhibition

Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Inhibition of miR-873 Provides Therapeutic Benefit in a Lipopolysaccharide-Induced Neuroinflammatory Model of Parkinson's Disease

doi: 10.1155/2020/8735249

Figure Lengend Snippet: The downregulation of ABCA1 by LPS via miR-873 in human glioblastoma U251 cells. The pre-miR-873 mRNA level was increased 4 h to 8 h after LPS treatment and returned to the control levels by 12 h (a); additionally, the miR-873 mRNA level was increased after LPS treatment for 12 h (b). The induction of miR-873 following LPS treatment for 24 h was eliminated by a TLR4 inhibitor (CLI-095) and a MyD88 inhibitor (ST2825) (c). The mRNA level of ABCA1 was increased 4 h after LPS treatment, but a significant decrease in ABCA1 was observed from 12 h to 24 h (d). The protein level of ABCA1 was reduced after LPS treatment for 24 h (e). Transfection of the miR-873 sponge decreased the mRNA levels of miR-873 (f). Transfection of the miR-873 sponge eliminated the increase in the ABCA1 mRNA levels 24 h after LPS treatment (g). The reduction in the ABCA1 levels was eliminated by a TLR4 inhibitor (CLI-095) and a MyD88 inhibitor (ST2825) following LPS treatment for 24 h (H). The data are expressed as the mean ± S.E.M.; n = 3, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 compared with the respective controls.

Article Snippet: These membranes were incubated with a polyclonal rabbit anti-human ABCA1 antibody (1 : 1000, NB400-105, Novus Biologicals, Colorado, USA) at 4°C overnight and then incubated with a biotinylated anti-rabbit IgG antibody (1 : 100, Vector Laboratories, Burlington, Canada).

Techniques: Control, Transfection

Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Inhibition of miR-873 Provides Therapeutic Benefit in a Lipopolysaccharide-Induced Neuroinflammatory Model of Parkinson's Disease

doi: 10.1155/2020/8735249

Figure Lengend Snippet: The regulation of ABCA1 expression by miR-873 in U251 cells. The luciferase activity in the cells transfected with the plasmid expressing the ABCA1 3′-untranslated region (3′-UTR) reporter was inhibited following cotransfection of the miR-873 expression vector (b). The predicted binding sites of miR-873 in the 3′-UTR of the human ABCA1 gene and the rodent Abca1 gene are shown (a). The mRNA (c) and protein (d) levels of ABCA1 were decreased following transfection with miR-873. The basal activity levels measured in the cells transfected with the empty vector were set to 1. The data are expressed as the mean ± S.E.M.; n = 3, ∗∗ p < 0.01 and ∗∗∗ p < 0.001 compared with the respective controls.

Article Snippet: These membranes were incubated with a polyclonal rabbit anti-human ABCA1 antibody (1 : 1000, NB400-105, Novus Biologicals, Colorado, USA) at 4°C overnight and then incubated with a biotinylated anti-rabbit IgG antibody (1 : 100, Vector Laboratories, Burlington, Canada).

Techniques: Expressing, Luciferase, Activity Assay, Transfection, Plasmid Preparation, Cotransfection, Binding Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Inhibition of miR-873 Provides Therapeutic Benefit in a Lipopolysaccharide-Induced Neuroinflammatory Model of Parkinson's Disease

doi: 10.1155/2020/8735249

Figure Lengend Snippet: The effects of ABCA1 silencing or miR-873 transfection on the levels of free cholesterol and α -synuclein in the lysosomes of normal SH-SY5Y cells or SH-SY5Y cells overexpressing α -synuclein. The levels of free cholesterol labeled with filipin (blue) in the lysosomes labeled with anti-LAMP-2 antibody (red) were increased in SH-SY5Y cells following ABCA1 silencing, as indicated by the colocalization coefficient (a and b). The lysosomal cholesterol was increased in SH-SY5Y cells following miR-873 transfection, as indicated by the colocalization coefficient (c and d). The distribution of α -synuclein (green) in the lysosomes labeled with anti-LAMP-2 antibody (red) was increased following ABCA1 silencing in SH-SY5Y cells overexpressing α -synuclein, as indicated by the colocalization coefficient (e and f). The levels of α -synuclein in the lysosomes were increased following miR-873 transfection in SH-SY5Y cells overexpressing α -synuclein, as indicated by the colocalization coefficient (g and h). The transfection of miR-873 reduced the mRNA levels of cathepsin D (CTSD) (i) and GCase (j) in SH-SY5Y cells overexpressing α -synuclein. The model of the disruption of intracellular cholesterol trafficking by miR-873 via ABCA1 in neuronal cells is shown (k). The data are expressed as the mean ± S.E.M.; n = 3, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 compared with the controls.

Article Snippet: These membranes were incubated with a polyclonal rabbit anti-human ABCA1 antibody (1 : 1000, NB400-105, Novus Biologicals, Colorado, USA) at 4°C overnight and then incubated with a biotinylated anti-rabbit IgG antibody (1 : 100, Vector Laboratories, Burlington, Canada).

Techniques: Transfection, Labeling, Disruption

Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Inhibition of miR-873 Provides Therapeutic Benefit in a Lipopolysaccharide-Induced Neuroinflammatory Model of Parkinson's Disease

doi: 10.1155/2020/8735249

Figure Lengend Snippet: The effects of miR-873 on autophagy in SH-SY5Y cells. The fluorescence of pEGFP-LC3 (green) was reduced in the cytoplasm of the cells (the nucleus was stained blue) transfected with miR-873 or sh-ABCA1 (a). The allosteric inhibitor of mTORC1, rapamycin, restored the miR-873-induced reduction in autophagic flux. The LC3II/LC3I protein expression ratio was reduced in the cells transfected with miR-873 or sh-ABCA1; moreover, the p62 protein accumulated in these cells (b). The data are expressed as the mean ± S.E.M.; n = 3, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 compared with the controls.

Article Snippet: These membranes were incubated with a polyclonal rabbit anti-human ABCA1 antibody (1 : 1000, NB400-105, Novus Biologicals, Colorado, USA) at 4°C overnight and then incubated with a biotinylated anti-rabbit IgG antibody (1 : 100, Vector Laboratories, Burlington, Canada).

Techniques: Fluorescence, Staining, Transfection, Expressing

Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Inhibition of miR-873 Provides Therapeutic Benefit in a Lipopolysaccharide-Induced Neuroinflammatory Model of Parkinson's Disease

doi: 10.1155/2020/8735249

Figure Lengend Snippet: The effects of sh-ABCA1 or sh-A20 transfection on DA neuron damage in a LPS-induced rat model of PD. The LPS-induced loss of DA neurons in the substantia nigra pars compacta of rats with ABCA1 (a) or A20 (e) knockdown was detected by immunohistochemistry staining ( n = 5). The percentage of tyrosine hydroxylase- (TH)- positive cells on the lesioned side relative to those on the intact side of rats with ABCA1 (b) or A20 (f) knockdown was calculated by ImageJ. The alteration of the mRNA levels of ABCA1 (c) and A20 (g) following LPS treatment ( n = 5). The number of rotations in the rats with ABCA1 (d) or A20 (h) knockdown 16 days after LPS injection ( n = 10). The data are expressed as the mean ± S.D.; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 compared with the controls.

Article Snippet: These membranes were incubated with a polyclonal rabbit anti-human ABCA1 antibody (1 : 1000, NB400-105, Novus Biologicals, Colorado, USA) at 4°C overnight and then incubated with a biotinylated anti-rabbit IgG antibody (1 : 100, Vector Laboratories, Burlington, Canada).

Techniques: Transfection, Knockdown, Immunohistochemistry, Staining, Injection

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: The sections stained with isotype matched normal rabbit IgG served as negative control (the control images of ABCA1 and SR-BI were in the or ). ( A ) Images stained with ABCA1 and SR-BI antibodies on Days 12, 15 and 18 of pregnency in Yorkshire pigs. The results show that the ABCA1 signals were observed mainly to LE at the mesometrial side and were barely detected in LE at the anti-mesometrial side. The positive signals were also barely detected in Tr and GE. The SR-BI signals were observed in LE, GE and Tr. The stain intensity of SR-BI in LE between at the mesometrial side and the anti-mesometrial side was similar. ( B ) Quantitative analysis of ABCA1 and SR-BI by measuring the average integrated optical density (IOD) in LE in Yorkshire pigs. Asterisks indicate significant differences (mean ± SD) between breeds (**P < 0.01), P value was determined by Student’s t test. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bars = 100 μm or 20 μm (amplification).

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control, Control, Amplification

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: ( A ) Images stained with ABCA1 and SR-BI antibodies on Days 12, 15 and 18 of pregnency in Meishan pigs. The expression patterns of ABCA1 and SR-BI both in Meishan pigs and Yorkshire pigs were similar. ( B ) Quantitative analysis of ABCA1 and SR-BI by measuring the average integrated optical density (IOD) in LE. Asterisks indicate significant differences (mean ± SD) between breeds (**P < 0.01), P value was determined by Student’s t test. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bars = 100 μm or 20 μm (amplification).

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Expressing, Amplification

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Positive signals of ABCA1 and SR-BI were shown in red and the blue staining represented nuclei (DAPI stained). The signals of ABCA1 and SR-BI were principally localized at the basolateral and apical side of the endometrial LE cell membrane. The sections stained with isotype matched normal rabbit IgG served as negative control (the control images of ABCA1 and SR-BI were in the or ). Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bar = 40 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Membrane, Negative Control, Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Uterine/placental sections were obtained on Days 26, 50 and 95 of pregnancy. Tissue sections were stained with rabbit anti-human ABCA1 polyclonal antibody.The sections stained with isotype matched normal rabbit IgG served as negative control. ( A ) Images were taken from the maternal-fetal interface in Yorkshire and Meishan pigs. ABCA1 positive cells were observed in the epithelial bilayer and GE in Yorkshire and Meishan pigs on Days 26, 50 and 95 of pregnancy. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; NC, negative control; Scale bar = 100 μm. ( B ) Quantitative analysis of ABCA1 by measuring the average integrated optical density (IOD) in the epithelial bilayer (composed by Tr and LE) and GE during pregnancy. Asterisks indicate significant differences (mean ± SD) between breeds (*P < 0.05; **P < 0.01. Analyzed by PROC MIXED of SAS). ( C ) Images were taken from the placental areolae in Yorkshire and Meishan pigs. The ABCA1 positive signals were filled in all the areolar regions, including Tr, LE and GE on Days 26, 50 and 95 of pregnancy. AR, areola; Scale bar = 100 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Positive staining of ABCA1 was shown in red and the staining was diffused in the positive cells (except the nuclei) without specific subcellular localization. The blue staining represents nuclei (DAPI stained). In Yorkshire pigs, the ABCA1 staining were mostly observed in LE on Days 26 and 50 of pregnancy, while on Day 95 of pregnancy, the positive signals were mostly observed in Tr and just a few positive signals could be observed in LE. In Meishan pigs, the ABCA1 staining was observed both in Tr and LE on Days 26, 50 and 95 of pregnancy. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; NC, negative control; Scale bar = 40 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: ( A ) schematic drawing of the epitheliochorial placenta of pig. ( B ) the maternal-fetal interface of the pig placenta composed of two regions, the interareolar region (composed by trophoblast and luminal epithelium, outside of arrowheads) and the areolar region (located at the mouth of uterine glands, enclosed by arrowheads), respectively. Our findings revealed two paths of cholesterol transport from maternal to fetal circulation, 1 the areolar-gland subunit; 2 the placental epithelial bilayer; ( C ) Schematic illustration of ABCA1 expression patterns in placental trophoblast in Yorkshire and Meishan pigs, showing that the ABCA1 (in red) was barely expressed in trophoblast which located at the interareolar region (outside of arrowheads) in Yorkshire, but it was highly expressed in the trophoblast in Meishan pigs. ( D ) Schematic illustration of the proposed mechanism for Meishan pig to increase the placental efficiency in transport cholesterol. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; AR, areola of placenta.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: The sections stained with isotype matched normal rabbit IgG served as negative control (the control images of ABCA1 and SR-BI were in the or ). ( A ) Images stained with ABCA1 and SR-BI antibodies on Days 12, 15 and 18 of pregnency in Yorkshire pigs. The results show that the ABCA1 signals were observed mainly to LE at the mesometrial side and were barely detected in LE at the anti-mesometrial side. The positive signals were also barely detected in Tr and GE. The SR-BI signals were observed in LE, GE and Tr. The stain intensity of SR-BI in LE between at the mesometrial side and the anti-mesometrial side was similar. ( B ) Quantitative analysis of ABCA1 and SR-BI by measuring the average integrated optical density (IOD) in LE in Yorkshire pigs. Asterisks indicate significant differences (mean ± SD) between breeds (**P < 0.01), P value was determined by Student’s t test. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bars = 100 μm or 20 μm (amplification).

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control, Amplification

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: ( A ) Images stained with ABCA1 and SR-BI antibodies on Days 12, 15 and 18 of pregnency in Meishan pigs. The expression patterns of ABCA1 and SR-BI both in Meishan pigs and Yorkshire pigs were similar. ( B ) Quantitative analysis of ABCA1 and SR-BI by measuring the average integrated optical density (IOD) in LE. Asterisks indicate significant differences (mean ± SD) between breeds (**P < 0.01), P value was determined by Student’s t test. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bars = 100 μm or 20 μm (amplification).

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Expressing, Amplification

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Positive signals of ABCA1 and SR-BI were shown in red and the blue staining represented nuclei (DAPI stained). The signals of ABCA1 and SR-BI were principally localized at the basolateral and apical side of the endometrial LE cell membrane. The sections stained with isotype matched normal rabbit IgG served as negative control (the control images of ABCA1 and SR-BI were in the or ). Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; Scale bar = 40 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Uterine/placental sections were obtained on Days 26, 50 and 95 of pregnancy. Tissue sections were stained with rabbit anti-human ABCA1 polyclonal antibody.The sections stained with isotype matched normal rabbit IgG served as negative control. ( A ) Images were taken from the maternal-fetal interface in Yorkshire and Meishan pigs. ABCA1 positive cells were observed in the epithelial bilayer and GE in Yorkshire and Meishan pigs on Days 26, 50 and 95 of pregnancy. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; NC, negative control; Scale bar = 100 μm. ( B ) Quantitative analysis of ABCA1 by measuring the average integrated optical density (IOD) in the epithelial bilayer (composed by Tr and LE) and GE during pregnancy. Asterisks indicate significant differences (mean ± SD) between breeds (*P < 0.05; **P < 0.01. Analyzed by PROC MIXED of SAS). ( C ) Images were taken from the placental areolae in Yorkshire and Meishan pigs. The ABCA1 positive signals were filled in all the areolar regions, including Tr, LE and GE on Days 26, 50 and 95 of pregnancy. AR, areola; Scale bar = 100 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: Positive staining of ABCA1 was shown in red and the staining was diffused in the positive cells (except the nuclei) without specific subcellular localization. The blue staining represents nuclei (DAPI stained). In Yorkshire pigs, the ABCA1 staining were mostly observed in LE on Days 26 and 50 of pregnancy, while on Day 95 of pregnancy, the positive signals were mostly observed in Tr and just a few positive signals could be observed in LE. In Meishan pigs, the ABCA1 staining was observed both in Tr and LE on Days 26, 50 and 95 of pregnancy. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; D, day of pregnancy; NC, negative control; Scale bar = 40 μm.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Staining, Negative Control

Journal: Scientific Reports

Article Title: Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency

doi: 10.1038/srep20503

Figure Lengend Snippet: ( A ) schematic drawing of the epitheliochorial placenta of pig. ( B ) the maternal-fetal interface of the pig placenta composed of two regions, the interareolar region (composed by trophoblast and luminal epithelium, outside of arrowheads) and the areolar region (located at the mouth of uterine glands, enclosed by arrowheads), respectively. Our findings revealed two paths of cholesterol transport from maternal to fetal circulation, 1 the areolar-gland subunit; 2 the placental epithelial bilayer; ( C ) Schematic illustration of ABCA1 expression patterns in placental trophoblast in Yorkshire and Meishan pigs, showing that the ABCA1 (in red) was barely expressed in trophoblast which located at the interareolar region (outside of arrowheads) in Yorkshire, but it was highly expressed in the trophoblast in Meishan pigs. ( D ) Schematic illustration of the proposed mechanism for Meishan pig to increase the placental efficiency in transport cholesterol. Legend: Tr, trophoblast; LE, endometrial luminal epithelium; GE, glandular epithelium; AR, areola of placenta.

Article Snippet: The sections were incubated with a rabbit anti-Human ABCA1 polyclonal antibody (1:300, Novus, NB400-105) or with a rabbit anti-Human SR-BI polyclonal antibody (1:300, Novus, NB400-104) at 4 °C overnight and then in biotinylated goat anti-rabbit secondary antibody (1:100, SA1022, Boster Corporation, China).

Techniques: Expressing

Journal: Clinical Epigenetics

Article Title: Hypermethylation of the TGF-β target, ABCA1 is associated with poor prognosis in ovarian cancer patients

doi: 10.1186/s13148-014-0036-2

Figure Lengend Snippet: ABCA1 expression and methylation level in IOSE cells and ovarian cancer cell lines. (A) Total RNA was isolated from ovarian cells and converted into cDNA for amplification with specific primers for ABCA1 . The relative level of expression after quantitative real-time RT-PCR was compared to IOSE cells (set as one fold). Each bar represents mean ± SD. (B) CP70 cells were treated with TSA (0.5 μM, 12 h), GSK343 (1 μM, 3 days), or 5aza (0.5 μM, 3 days). The expression level of ABCA1 was determined by RT-PCR. Treatment of 5aza, but not TSA or GSK, resulted in robust re-expression of ABCA1 in CP70 cells. Each bar represents mean ± SD. (C) The methylation status of the ABCA1 promoter and TSS region was analyzed by bisulfite pyro-sequencing from −90 to +190 (black line underneath). The upper panel shows the ABCA1 promoter and TSS region and the corresponding CpG sites (vertical bar), and the lower panel illustrates DNA methylation at the interrogated CpG site (circle) in IOSE cells, two NOSE samples, and ovarian cancer cell lines with intensity of gray color indicating methylation level.

Article Snippet: The immunohistochemistry procedure followed a standard protocol, using a rabbit polyclonal anti-human ABCA1 antibody (NB400-105, Novus Biologicals).

Techniques: Expressing, Methylation, Isolation, Amplification, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Sequencing, DNA Methylation Assay

Journal: Clinical Epigenetics

Article Title: Hypermethylation of the TGF-β target, ABCA1 is associated with poor prognosis in ovarian cancer patients

doi: 10.1186/s13148-014-0036-2

Figure Lengend Snippet: Effects of ABCA1 knockdown on cell growth. Real-time RT-PCR expression of ABCA1 in (A) MCP3 and (B) HeyC2 cells infected by lentivirus against shGFP (control) or shABCA1. Each bar represents mean ± SD. The growth of ABCA1 knockdown (C) MCP3 and (D) HeyC2 cells was examined by soft agar assay. Quantitative analysis of the soft agar assay is also shown. *** P < 0.001; * P < 0.05.

Article Snippet: The immunohistochemistry procedure followed a standard protocol, using a rabbit polyclonal anti-human ABCA1 antibody (NB400-105, Novus Biologicals).

Techniques: Knockdown, Quantitative RT-PCR, Expressing, Infection, Control, Soft Agar Assay

Journal: Clinical Epigenetics

Article Title: Hypermethylation of the TGF-β target, ABCA1 is associated with poor prognosis in ovarian cancer patients

doi: 10.1186/s13148-014-0036-2

Figure Lengend Snippet: Effects of ABCA1 knockdown on cholesterol level and ovarian cancer growth in vivo . The cholesterol level of ABCA1 knockdown (A) MCP3 and (B) HeyC2 cells was measured by cholesterol quantitation assay. (C) The effect of ABCA1 knockdown on tumor growth in vivo was determined by the nude mice model. HeyC2 cells stably infected with shABCA1 (red arrow) or shGFP (green arrow) were injected subcutaneously into athymic nude mice. One week later, tumor volumes were measured daily. From day 19, the volume of tumors with ABCA1 knockdown was significantly reduced as compared to vector controls (* P < 0.05; ** P < 0.005). Data were expressed as mean ± SD ( n = 3).

Article Snippet: The immunohistochemistry procedure followed a standard protocol, using a rabbit polyclonal anti-human ABCA1 antibody (NB400-105, Novus Biologicals).

Techniques: Knockdown, In Vivo, Quantitation Assay, Stable Transfection, Infection, Injection, Plasmid Preparation

Journal: Clinical Epigenetics

Article Title: Hypermethylation of the TGF-β target, ABCA1 is associated with poor prognosis in ovarian cancer patients

doi: 10.1186/s13148-014-0036-2

Figure Lengend Snippet: Association between methylation of ABCA1 and clinicopathological features of 76 ovarian cancer patients

Article Snippet: The immunohistochemistry procedure followed a standard protocol, using a rabbit polyclonal anti-human ABCA1 antibody (NB400-105, Novus Biologicals).

Techniques: Methylation

Journal: Clinical Epigenetics

Article Title: Hypermethylation of the TGF-β target, ABCA1 is associated with poor prognosis in ovarian cancer patients

doi: 10.1186/s13148-014-0036-2

Figure Lengend Snippet: Association between ABCA1 methylation and tumor progression. Dot plot showing the association between ABCA1 methylation in different (A) stages and (B) grades in 76 ovarian cancer patient samples. Methylation of ABCA1 was determined by bisulfite pyro-sequencing. Low stage and low grade represented FIGO I and II and grade 1–2, respectively. While high stage and high grade represented FIGO III and IV and grade 3, respectively. * P < 0.05 by the Mann-Whitney U test. Kaplan-Meier analysis of ABCA1 methylation for (C) progression-free survival and (D) overall survival in 76 ovarian cancer patient samples is shown. Patients were grouped according to methylation of ABCA1 of 3%, which is based on the methylation level of IOSE cells. Patients with “high” ABCA1 methylation (>3% methylation) have significant shorter overall survival ( P = 0.019) but not progression-free survival than patients with “low” ABCA1 methylation. Log-rank P values are shown.

Article Snippet: The immunohistochemistry procedure followed a standard protocol, using a rabbit polyclonal anti-human ABCA1 antibody (NB400-105, Novus Biologicals).

Techniques: Methylation, Sequencing, MANN-WHITNEY

Journal: Clinical Epigenetics

Article Title: Hypermethylation of the TGF-β target, ABCA1 is associated with poor prognosis in ovarian cancer patients

doi: 10.1186/s13148-014-0036-2

Figure Lengend Snippet: Univariable analysis of survival by the Cox proportional hazards model

Article Snippet: The immunohistochemistry procedure followed a standard protocol, using a rabbit polyclonal anti-human ABCA1 antibody (NB400-105, Novus Biologicals).

Techniques: Methylation

Journal: Clinical Epigenetics

Article Title: Hypermethylation of the TGF-β target, ABCA1 is associated with poor prognosis in ovarian cancer patients

doi: 10.1186/s13148-014-0036-2

Figure Lengend Snippet: Multivariate analysis of survival by the Cox proportional hazards model

Article Snippet: The immunohistochemistry procedure followed a standard protocol, using a rabbit polyclonal anti-human ABCA1 antibody (NB400-105, Novus Biologicals).

Techniques: Methylation

Journal: Clinical Epigenetics

Article Title: Hypermethylation of the TGF-β target, ABCA1 is associated with poor prognosis in ovarian cancer patients

doi: 10.1186/s13148-014-0036-2

Figure Lengend Snippet: Association between expression of ABCA1 and survival in ovarian cancer patients. Expression of ABCA1 in 55 ovarian cancer patient samples was determined by IHC in tissue microarray. (A) Representative image of ovarian cancer showing high (left panel) and low (right panel) ABCA1 expression on the cell membrane or cytoplasm (×400). (B) Kaplan-Meier analysis found that patients with low ABCA1 expression have shorter progression-free survival than patients with high ABCA1 expression ( P = 0.038). (C) Similar results can be observed in TCGA ovarian cancer RNA-Seq dataset that patients with low expression of ABCA1 are associated with shorter overall survival ( P = 0.0008). Log-rank P values are shown.

Article Snippet: The immunohistochemistry procedure followed a standard protocol, using a rabbit polyclonal anti-human ABCA1 antibody (NB400-105, Novus Biologicals).

Techniques: Expressing, Microarray, Membrane, RNA Sequencing

Journal: Clinical Epigenetics

Article Title: Hypermethylation of the TGF-β target, ABCA1 is associated with poor prognosis in ovarian cancer patients

doi: 10.1186/s13148-014-0036-2

Figure Lengend Snippet: Association between expression of ABCA1 and clinicopathological features of 55 ovarian cancer patients

Article Snippet: The immunohistochemistry procedure followed a standard protocol, using a rabbit polyclonal anti-human ABCA1 antibody (NB400-105, Novus Biologicals).

Techniques: Expressing